Coding

Part:BBa_K2957094:Design

Designed by: Ye Cheng Zheng   Group: iGEM19_MIT   (2019-10-19)


C5a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3031
    Illegal SpeI site found at 2143
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3031
    Illegal SpeI site found at 2143
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3031
    Illegal BglII site found at 992
    Illegal XhoI site found at 1108
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3031
    Illegal SpeI site found at 2143
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3031
    Illegal SpeI site found at 2143
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2644


Design Notes

We used Modular Cloning Golden Gate Type II Assembly, so we had to consider how parts came together. We also used the natural secretion tag given in the part so we could use it to test chemokine secretion success by HEKs. See more on the MIT iGEM 2019 wiki: https://2019.igem.org/Team:MIT.

Source

This part was ordered from IDT/Twist Biosciences as a gBlock and then assembled into a plasmid. The sequence was taken from this site: https://www.uniprot.org/uniprot/P01031 and then codon-optimized using IDT's codon optimization tool.

References